ObjectiveViral infections account for 15–20% of human cancers worldwide.The potential roles of various viruses, such as polyomaviruses (PyV), human cytomegalovirus (HCMV) and human papillomavirus (HPV) in brain tumors remain unclear. The aim of this study was investigation of the prevalence of different viruses in different types of brain tumors including glioblastoma multiform (GBM), astrocytoma, oligodendroglioma, meningioma and B cell lymphoma. Meanwhile we described the relation of age/gender and cancer distribution.Methods86 formalin-fixed paraffin-embedded samples have examined for the prevalence of JCPyV, BKPyV, HCMV and HPV in different brain tumors. We tested JCPyV, BKPyV by using Real Time PCR targeting the large T-antigen and H

Objectives: The aim of present work was to assess cytomegalovirus (CMV) viremia in Iranian human immunodeficiency virus (HIV)-1-infected patients with a CD4+ count< 100 cells/mm 3 and to explore whether CMV DNA loads correlate with CD4+ cell counts or associated retinitis. Methods: This study was conducted at the AIDS research center in Iran on HIV-1-infected patients with CD4+ count< 100 cells/mm 3, antiretroviral therapy-naive, aged≥ 18 years with no previous history of CMV end-organ disease (CMV-EOD). Results: Thirty-nine of 82 patients (47.56%) had detectable CMV viral load ranging from 66 to 485,500 IU/mL. CMV viral load in patients with retinitis ranges from 352 to 2,720 IU/mL, and it was undetectable in 2 patients. No significant a

Aim: We conducted a case–control study to examine the presence of BK virus (BKV) and Epstein barr virus (EBV) genome in prostate specimens of benign prostatic hyperplasia (BPH) and prostate cancer (PCa) in Iran. Materials & methods: 64 paraffin-embedded PCa specimens were included as case subjects and 57 paraffin-embedded BPH specimens as control. DNA extraction was carried out by phenol–chloroform technique and PCR for detection of BKV and EBV. Results: 90 (73%) out of 121 specimens were collected by prostatectomy and 31 (27%) by transurethral resection of the prostate. BKV was not detected in any of the samples and one positive EBV was reported. Conclusion: These findings provide further evidence against a causative role of BKV and EB

In Jan 2020, the outbreak of the 2019 novel coronavirus (SARS-CoV-2) in Wuhan, Hubei Province of China spread increasingly to other countries worldwide which WHO declared it as a public health emergency of international concern. Iran was included in the affected countries. Throat swab specimens were collected and tested by using real-time reverse transcription PCR (RT-PCR) kit targeting the E region for screening and RNA dependent RNA polymerase for confirmation. Conventional RT-PCR was conducted for the N region and the PCR products were sequenced by Sanger sequencing. The first seven cases of SARS-CoV-2 infections were identified in Qom, Iran. This report describes the clinical and epidemiological features of the first cases of SARS-CoV-2

Aim: The aim of the present study was to investigate the prevalence and genetic variation of the human metapneumovirus (HMPV) G gene in children less than 8 years old with acute respiratory tract infections in Iran during 2014–2015. Materials & methods: To this end, 110 nasal swabs were tested for HMPV F gene. All positive samples were tested for the full length of HMPV G gene, then sequenced and analyzed for phylogenetic grouping. Results: Six (5.45%) out of 110 samples were positive for HMPV gene. Our HMPV sequences clustered into two main clusters: A2b (five isolates) and B1 (one isolate). The dN/dS ratio showed the presence of negative selective pressure. Conclusion: A2b was the predominant genotype during the study period in southwes

BackgroundMiscarriage is the spontaneous pregnancy loss before 24 wk of gestation. The incidence rate of miscarriage over the past few decades has shown steady or even growing trends. Viral intrauterine infections are one of the probable etiological causes of miscarriage. Previous evidence have shown that human herpes viruses (HHVs) could be considered as the potential reasons for intrauterine infections and adverse pregnancy outcomes.ObjectiveThis case-control study aimed to detect HHV1-5 DNAs in placental tissues and assess their association with miscarriage during the first 24 wk of pregnancy in spontaneous and therapeutic abortions.Materials and MethodsPlacental tissues from 83 women with spontaneous abortions during the first and the s

Aim: We investigated the genetic characterization of the respiratory syncytial virus (RSV) ON-1 genotypes and their different lineages based on the G gene among children< 2 years of age presenting with acute respiratory tract infections in Tehran, Iran. Materials & methods: A phylogenetic tree from the Iranian samples and ON-1 strains of various parts of the world were constructed. The amino acid composition of the RSV G protein of the ON-1 genotype was mapped. Results: Human RSV ON-1 genotypes from the Iranian samples clustered in three lineages. The most common amino acid substitutions were as follows: X218Q, I240S, L289P, Y304H and L310P. Conclusion: Continuing molecular epidemiological surveys in other regions of Iran will provide deepe

Aims: Human herpes virus 6 (HHV-6) is a ubiquitous virus with a high rate of prevalence worldwide. In recent years, a new form of the virus genome has been identified called Chromosomally integrated HHV-6 (ciHHV-6). Further studies are required to elucidate the relation between ciHHV-6 and other clinical complications. The purpose of this study was to summarize the literature on different clinical aspects of the ciHHV-6 integration and its prevalevce in different communities.Materials and methods: Search keywords include HHV-6, integrated genome, telomeric regions and integrated HHV-6. Scientific databases such as scopus, PubMed and google scholar with no specified start date were used for information collection.Findings: About 1% of global